# Microbial eukaryotic focused metatranscriptome data # PI: David Caron # Data version 2: 2020-02-26 ========================= SRA_run SRA_run_link SRA_study bioproject_accession biosample_accession library_ID title sample_name library_strategy library_source library_selection library_layout platform instrument_model design_description filetype filename filename2 ------------------------- SRR5799338 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799338 SRP110974 PRJNA391503 SAMN07269826 SPOT_surface_7 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_7_CAGATC_L002_R1_001.fastq.gz SPOT_surface_7_CAGATC_L002_R2_001.fastq.gz SRR5799339 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799339 SRP110974 PRJNA391503 SAMN07269827 SPOT_surface_8 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_8_ACTTGA_L002_R1_001.fastq.gz SPOT_surface_8_ACTTGA_L002_R2_001.fastq.gz SRR5799336 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799336 SRP110974 PRJNA391503 SAMN07269828 SPOT_surface_9 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_9_GATCAG_L002_R1_001.fastq.gz SPOT_surface_9_GATCAG_L002_R2_001.fastq.gz SRR5799337 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799337 SRP110974 PRJNA391503 SAMN07269829 SPOT_surface_10 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_10_TAGCTT_L002_R1_001.fastq.gz SPOT_surface_10_TAGCTT_L002_R2_001.fastq.gz SRR5799334 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799334 SRP110974 PRJNA391503 SAMN07269830 SPOT_surface_11 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_11_GGCTAC_L002_R1_001.fastq.gz SPOT_surface_11_GGCTAC_L002_R2_001.fastq.gz SRR5799335 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799335 SRP110974 PRJNA391503 SAMN07269831 SPOT_surface_12 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_12_CTTGTA_L002_R1_001.fastq.gz SPOT_surface_12_CTTGTA_L002_R2_001.fastq.gz SRR5799332 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799332 SRP110974 PRJNA391503 SAMN07269832 SPOT_150m_25 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_150m_25_ATCACG_L005_R1_001.fastq.gz SPOT_150m_25_ATCACG_L005_R2_001.fastq.gz SRR5799333 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799333 SRP110974 PRJNA391503 SAMN07269833 SPOT_150m_26 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_150m_26_CGATGT_L005_R1_001.fastq.gz SPOT_150m_26_CGATGT_L005_R2_001.fastq.gz SRR5799340 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799340 SRP110974 PRJNA391503 SAMN07269834 SPOT_150m_27 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_150m_27_TTAGGC_L005_R1_001.fastq.gz SPOT_150m_27_TTAGGC_L005_R2_001.fastq.gz SRR5799341 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799341 SRP110974 PRJNA391503 SAMN07269835 SPOT_890m_28 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_890m_28_TGACCA_L006_R1_001.fastq.gz SPOT_890m_28_TGACCA_L006_R2_001.fastq.gz SRR5799343 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799343 SRP110974 PRJNA391503 SAMN07269836 SPOT_890m_29 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_890m_29_ACAGTG_L006_R1_001.fastq.gz SPOT_890m_29_ACAGTG_L006_R2_001.fastq.gz SRR5799344 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799344 SRP110974 PRJNA391503 SAMN07269837 SPOT_890m_30 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_890m_30_GCCAAT_L006_R1_001.fastq.gz SPOT_890m_30_GCCAAT_L006_R2_001.fastq.gz SRR5799342 https://trace.ncbi.nlm.nih.gov/Traces/sra/?run=SRR5799342 SRP110974 PRJNA391503 SAMN07269838 SPOT_890m_31 SPOT metatranscriptome survey - vertical profile; polyA tail selection nd RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_890m_31_CAGATC_L006_R1_001.fastq.gz SPOT_890m_31_CAGATC_L006_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_19_S22_L004 Metatranscriptome survey S. CA surface Catalina_19 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_19_S22_L004_R1_001.fastq.gz Catalina_19_S22_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_20_S23_L004 Metatranscriptome survey S. CA surface Catalina_20 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_20_S23_L004_R1_001.fastq.gz Catalina_20_S23_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_21_S24_L004 Metatranscriptome survey S. CA surface Catalina_21 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_21_S24_L004_R1_001.fastq.gz Catalina_21_S24_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_22_S25_L004 Metatranscriptome survey S. CA surface Catalina_22 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_22_S25_L004_R1_001.fastq.gz Catalina_22_S25_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_23_S26_L004 Metatranscriptome survey S. CA surface Catalina_23 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_23_S26_L004_R1_001.fastq.gz Catalina_23_S26_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Catalina_24_S27_L004 Metatranscriptome survey S. CA surface Catalina_24 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Catalina_24_S27_L004_R1_001.fastq.gz Catalina_24_S27_L004_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_1_S1_L001 Metatranscriptome survey S. CA surface Port_of_LA_1 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_1_S1_L001_R1_001.fastq.gz Port_of_LA_1_S1_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_2_S2_L001 Metatranscriptome survey S. CA surface Port_of_LA_2 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_2_S2_L001_R1_001.fastq.gz Port_of_LA_2_S2_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_3_S3_L001 Metatranscriptome survey S. CA surface Port_of_LA_3 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_3_S3_L001_R1_001.fastq.gz Port_of_LA_3_S3_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_4_S4_L001 Metatranscriptome survey S. CA surface Port_of_LA_4 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_4_S4_L001_R1_001.fastq.gz Port_of_LA_4_S4_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_5_S5_L001 Metatranscriptome survey S. CA surface Port_of_LA_5 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_5_S5_L001_R1_001.fastq.gz Port_of_LA_5_S5_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd Port_of_LA_6_S6_L001 Metatranscriptome survey S. CA surface Port_of_LA_6 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq Port_of_LA_6_S6_L001_R1_001.fastq.gz Port_of_LA_6_S6_L001_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_13_S15_L003 Metatranscriptome survey S. CA surface SPOT_surface_13 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_13_S15_L003_R1_001.fastq.gz SPOT_surface_13_S15_L003_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_14_S16_L003 Metatranscriptome survey S. CA surface SPOT_surface_14 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_14_S16_L003_R1_001.fastq.gz SPOT_surface_14_S16_L003_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_15_S17_L003 Metatranscriptome survey S. CA surface SPOT_surface_15 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_15_S17_L003_R1_001.fastq.gz SPOT_surface_15_S17_L003_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_16_S18_L003 Metatranscriptome survey S. CA surface SPOT_surface_16 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_16_S18_L003_R1_001.fastq.gz SPOT_surface_16_S18_L003_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_17_S19_L003 Metatranscriptome survey S. CA surface SPOT_surface_17 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_17_S19_L003_R1_001.fastq.gz SPOT_surface_17_S19_L003_R2_001.fastq.gz nd nd nd PRJNA608423 nd SPOT_surface_18_S20_L003 Metatranscriptome survey S. CA surface SPOT_surface_18 RNA-Seq METATRANSCRIPTOMIC RT-PCR paired ILLUMINA Illumina HiSeq 2500 Total RNA extraction; polyA tail selection of eukaryotic mRNA; cDNA library preparation fastq SPOT_surface_18_S20_L003_R1_001.fastq.gz SPOT_surface_18_S20_L003_R2_001.fastq.gz